Volume 241, Issue 5 p. 2143-2157
Full paper

COG3 confers the chilling tolerance to mediate OsFtsH2-D1 module in rice

Dongfeng Liu

Dongfeng Liu

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China

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Shengtao Luo

Shengtao Luo

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China

University of Chinese Academy of Sciences, Beijing, 100049 China

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Zhitao Li

Zhitao Li

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China

University of Chinese Academy of Sciences, Beijing, 100049 China

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Guohua Liang

Guohua Liang

Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou, 225009 China

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Yalong Guo

Yalong Guo

State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China

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Yunyuan Xu

Yunyuan Xu

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China

University of Chinese Academy of Sciences, Beijing, 100049 China

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Kang Chong

Corresponding Author

Kang Chong

Key Laboratory of Plant Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, 100093 China

University of Chinese Academy of Sciences, Beijing, 100049 China

Author for correspondence:

Kang Chong

Email: [email protected]

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First published: 03 January 2024

Summary

  • The chilling stress induced by the global climate change harms rice production, especially at seedling and booting stage, which feed half the population of the world. Although there are key quantitative trait locus genes identified in the individual stage, few genes have been reported and functioned at both stages.
  • Utilizing chromosome segment substitution lines (CSSLs) and a combination of map-based cloning and phenotypes of the mutants and overexpression lines, we identified the major gene Chilling-tolerance in Geng/japonica rice 3 (COG3) of q chilling-tolerance at the booting and seedling stage 11 (qCTBS11) conferred chilling tolerance at both seedling and booting stages.
  • COG3 was significantly upregulated in Nipponbare under chilling treatment compared with its expression in 93-11. The loss-of-function mutants cog3 showed a reduced chilling tolerance. On the contrary, overexpression enhanced chilling tolerance. Genome evolution and genetic analysis suggested that COG3 may have undergone strong selection in temperate japonica during domestication. COG3, a putative calmodulin-binding protein, physically interacted with OsFtsH2 at chloroplast. In cog3-1, OsFtsH2-mediated D1 degradation was impaired under chilling treatment compared with wild-type.
  • Our results suggest that COG3 is necessary for maintaining OsFtsH2 protease activity to regulate chilling tolerance at the booting and seedling stage.

Data availability

The data that support the findings of this study are available in the Supporting Information of this article.