Knockout of the sugar transporter OsSTP15 enhances grain yield by improving tiller number due to increased sugar content in the shoot base of rice (Oryza sativa L.)
Mingjuan Li
College of Resources, Hunan Agricultural University, Changsha, 410128 China
These authors contributed equally to this work.
Search for more papers by this authorHongye Li
College of Resources, Hunan Agricultural University, Changsha, 410128 China
These authors contributed equally to this work.
Search for more papers by this authorQidong Zhu
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorDong Liu
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorZhen Li
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorHaifei Chen
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorJinsong Luo
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorPan Gong
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorAbdelbagi M. Ismail
Crop and Environmental Sciences Division, International Rice Research Institute, Metro Manila, 1301 Philippines
Search for more papers by this authorCorresponding Author
Zhenhua Zhang
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Yuelushan Laboratory, Hongqi Road, Changsha, Hunan, 410128 China
Author for correspondence:
Zhenhua Zhang
Email: [email protected]
Search for more papers by this authorMingjuan Li
College of Resources, Hunan Agricultural University, Changsha, 410128 China
These authors contributed equally to this work.
Search for more papers by this authorHongye Li
College of Resources, Hunan Agricultural University, Changsha, 410128 China
These authors contributed equally to this work.
Search for more papers by this authorQidong Zhu
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorDong Liu
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorZhen Li
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorHaifei Chen
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorJinsong Luo
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorPan Gong
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Search for more papers by this authorAbdelbagi M. Ismail
Crop and Environmental Sciences Division, International Rice Research Institute, Metro Manila, 1301 Philippines
Search for more papers by this authorCorresponding Author
Zhenhua Zhang
College of Resources, Hunan Agricultural University, Changsha, 410128 China
Yuelushan Laboratory, Hongqi Road, Changsha, Hunan, 410128 China
Author for correspondence:
Zhenhua Zhang
Email: [email protected]
Search for more papers by this authorSummary
- Sugar transporter proteins (STPs) play critical roles in regulating plant stress tolerance, growth, and development. However, the role of STPs in regulating crop yield is poorly understood.
- This study elucidates the mechanism by which knockout of the sugar transporter OsSTP15 enhances grain yield via increasing the tiller number in rice.
- We found that OsSTP15 is specifically expressed in the shoot base and vascular bundle sheath of seedlings and encodes a plasma membrane-localized high-affinity glucose efflux transporter. OsSTP15 knockout enhanced sucrose and trehalose-6-phosphate (Tre6P) synthesis in leaves and improved sucrose transport to the shoot base by inducing the expression of sucrose transporters. Higher glucose, sucrose, and Tre6P contents were observed at the shoot base of stp15 plants. Transcriptome and metabolome analyses of the shoot base demonstrated that OsSTP15 knockout upregulated the expression of cytokinin (CK) synthesis- and signaling pathway-related genes and increased CK levels.
- These findings suggest that OsSTP15 knockout represses glucose export from the cytoplasm and simultaneously enhances sugar transport from source leaves to the shoot base by promoting the synthesis of sucrose and Tre6P in leaves. Subsequent accumulation of glucose, sucrose, and Tre6P in the shoot base promotes tillering by stimulating the CK signaling pathway.
Open Research
Data availability
The data that support the findings of this study are available in the Supporting Information.
Supporting Information
| Filename | Description |
|---|---|
| nph19411-0001-DatasetS1.xlsxExcel 2007 spreadsheet , 42.8 KB |
Dataset S1 Differentially expressed genes in the shoot base of stp15 and ZH11 seedlings. |
| nph19411-0002-SupInfo.pdfPDF document, 2.9 MB |
Fig. S1 Co-expression analysis of OsFON1 and the relative expression of OsFON1 in the shoot base. Fig. S2 Comparison of the agronomic trait of wild-type ZH11 and stp15 lines. Fig. S3 Sugar concentration in different tissues of wild-type ZH11 and stp15 plants at 24 d after pollination. Fig. S4 Promoter-GUS analysis and RNA in situ hybridization of OsSTP15. Fig. S5 Regulation of OsSTP15 expression by sugars. Fig. S6 Phylogenetic tree and transmembrane domains prediction of OsSTP15. Fig. S7 Multiple sequence alignment of the OsSTP15 with other STPs in rice and Arabidopsis. Fig. S8 Sugar transport assay of OsSTP15. Fig. S9 Photosynthesis rate and the relative expression level of sugar transporter genes in ZH11 and stp15 leaves. Fig. S10 Knockout of OsSTP15 and feeding glucose or sucrose promote tillering in rice. Fig. S11 RNA-seq analysis of the shoot base of 30-d-old wild-type ZH11 and stp15 seedlings. Fig. S12 Effects of STP15 knockout on auxin concentration and relative expression level of genes related to auxin pathway in the shoot base. Fig. S13 Analysis of differentially expressed genes associated with cell wall component. Fig. S14 Effect of sucrose feeding and cytokinin synthesis inhibitor lovastatin treatments on rice tillering. Methods S1 Supplemental material and methods. Table S1 Nucleotide sequences of all primers used in this study. Table S2 Grain yield-related agronomic traits of T1 generation stp15 and wild-type. Table S3 Concentrations of GA and SL in shoot base of wild-type and stp15. Please note: Wiley is not responsible for the content or functionality of any Supporting Information supplied by the authors. Any queries (other than missing material) should be directed to the New Phytologist Central Office. |
Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
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